Kinetic study of anti-viral ribavirin uptake mediated by hCNT3 and hENT1 in Xenopus laevis oocytes
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چکیده
منابع مشابه
Kinetic study of anti-viral ribavirin uptake mediated by hCNT3 and hENT1 in Xenopus laevis oocytes.
Transport across the cell membrane is crucial in drug delivery. However, the process is complicated because nucleoside derivatives that are commonly used as anti-viral drugs are transported through two different types of specific transporters: concentrative transporters and equilibrative transporters. Cross-disciplinary approaches involving both biological experiments and theoretical considerat...
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RNA from encephalomyocarditis virus was injected into oocytes of Xenopus laevis. After incubation of the oocytes in [(35)S]methionine, polyacrylamide gel electrophoresis showed that a new series of polypeptides had been synthesized. They were identical in size to the polypeptides that appeared in ascites cells after infection with this virus. Electrophoretic and chromatographic analysis of the ...
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The amiloride-sensitive Na + /H + antiporter in defolliculated oocytes of Xenopus laevis was characterized by measurements of H Na + influx and apparent H + efflux. Uptake of ^Na"" was linear over a 90-min incubation period and was inhibited approximately 80% with 5xlO~moll~ amiloride. Amiloride-sensitive sodium uptake was reduced following collagenase treatment or oocyte aging. Ko 5 for amilor...
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Microinjection of Xenopus laevis oocytes followed by thin-sectioning electron microscopy (EM) is an excellent system for studying nucleocytoplasmic transport. Because of its large nucleus and high density of nuclear pore complexes (NPCs), nuclear transport can be easily visualized in the Xenopus oocyte. Much insight into the mechanisms of nuclear import and export has been gained through use of...
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ژورنال
عنوان ژورنال: Biophysical Chemistry
سال: 2010
ISSN: 0301-4622
DOI: 10.1016/j.bpc.2009.12.012